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Valery V. Khramtsov, PhD
Associate Professor
201 Davis Heart and Lung Research Institute
473 West 12th Avenue
Columbus, OH 43210
Phone: (614) 247-7707
Education
M.S. Novosibirsk State University, Novosibirsk, Russia
Ph.D. Institute of Chemical Kinetics & Combustion, Novosibirsk, Russia
Dr.Sci. Institute of Catalysis, Novosibirsk, Russia
Research Interests
Dr. Khramtsov’s research interests include the wide range of Magnetic Resonance applications in chemistry and biology, and studies of free radical processes related to physiological and pathophysiological functions of reactive oxygen (ROS), reactive nitrogen (RNS) and reactive sulfur (RSS) species in various biological systems and models of oxidative stress.
Specific areas of current focus of Dr. Khramtsov’s laboratory:
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Functional EPR spectroscopy and imaging, including in vitro and in vivo applications, based on the development of new probes (among them pH-, SH- and NO-sensitive nitroxides)
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NMR Spin Trapping technique development and applications, using unique NMR-sensitive traps, to the study of EPR silent chemistry of the radical adducts and ROS detection in biological models of oxidative stress
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Studies of redox chemistry of the nitroxides, nitrones and oxidant-sensitive fluorescent probes related to the mechanisms of their reaction with ROS and to their antioxidant properties.
Honors
1987 Award of All-Union Mendeleev Chemical Society (USSR)
1989 Research Fellowship of Alexander von Humboldt Foundation (Germany)
1994 Gold Medal, State Award of Russian Federation in Science and Technology (presented by President of Russia)
1997 Research Fellowship of Fogarty International Center (NIH, USA)
Professional Societies
1990–present International EPR Society
1998–present Society for Free Radical Biology and Medicine (former the Oxygen Society)
Recent selected publications
Reviews
1. Swartz HM, Khan N, and Khramtsov VV. 2007, Use of Electron Paramagnetic Resonance Spectroscopy to Evaluate the Redox State In Vivo, Antioxid. Redox Signal. 9 (10), 1757-1771.
2. Khramtsov, V.V. Biological Imaging and Spectroscopy of pH. 2005, Current Organic Chemistry, 9, 909-923.
3. Khramtsov VV, Grigor'ev IA, Foster MA and Lurie DJ. 2004, In Vitro and In Vivo Measurement of pH and Thiols by EPR-based Techniques, Antioxid. Redox Signal. 6 (3), 667-676.
Papers
1. Roshchupkina GI, Bobko AA, Bratasz A, Reznikov VA, Kuppusamy P, and Khramtsov VV. 2008, In vivo EPR measurement of glutathione in tumor-bearing mice using improved disulfide biradical probe, Free Radic. Biol. Medicine, 45, 312-320.
2. Bobko AA, Dhimitruka I, Zweier JL, Khramtsov VV. 2007, Trityl radicals as persistent dual function pH and oxygen probes for in vivo electron paramagnetic resonance spectroscopy and imaging: concept and experiment, J. Am. Chem. Soc. 129 (23), 7240-7241.
3. Bobko AA, Kirilyuk IA, Grigor’ev IA, Zweier JL and Khramtsov VV. 2007, Reversible Reduction of Nitroxides to Hydroxylamines: the Roles for Ascorbate and Glutathione, Free Radic. Biol. Medicine, 42, 404-412.
4. Samouilov A, Zweier JL, Woldman Y, and Khramtsov VV. 2007, Magnetic Resonance Study of the Transmembrane Nitrite Diffusion, Nitric Oxide; 16, 362-370.
5. Potapenko DI, Foster MA, Kirilyuk IA, Lurie DJ, Hutchitson JMS, Grigor’ev IA, Bagryanskaya EG, and Khramtsov VV. 2006, Real-time monitoring of drug-induced changes in the stomach acidity of living rats using improved pH-sensitive nitroxides and low-field EPR techniques, J. Magn. Reson. 182, 1-11.
6. Glazachev YI, and Khramtsov VV. Fluorescence recovery under decaying photobleaching irradiation: concept and experiment. J. Fluoresc. 2006, 16 (6), 773-781.
7. Komarov DA, Slepneva IA, Glupov VV, and Khramtsov VV. 2005, Superoxide and Hydrogen Peroxide Formation during Enzymatic Oxidation of DOPA by Phenoloxidase, Free Radic. Res. 39 (8): 853-858.
8. Potapenko DI, Bagryanskaya EG, Grigoriev IA, Maksimov AM, Reznikov VA, Platonov VE, Clanton TL, and Khramtsov VV. 2005, Quantitative determination of SH-groups using 19F NMR spectroscopy and disulphide of 2,3,5,6-tetrafluoro-4-mercapto-benzoic acid, Magn. Reson. Chem., 43 (11), 902-909.
9. Bobko AA, Bagryanskaya EG, Reznikov VA, Kolosova NG, Clanton TL, and Khramtsov VV. 2004 Redox Sensitive Mechanism of NO Scavenging by Nitronyl Nitroxides, Free Rad.Biol.Med. 36/2, 248-258.
10. Potapenko DI, Bagryanskaya EG, Tsentalovich YP, Reznikov VV, Clanton TL and Khramtsov VV. 2004, Reversible reaction of thiols and thiyl radicals with nitrone spin traps, J. Phys. Chem. B., 108, 9315-9324.
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